Effects of R-(1-amino-2-phenylethyl)phosphonic acid on glyceollin accumulation and expression of resistance to Phytophthora megasperma f.sp. glycinea in soybean.
Identifieur interne : 002D81 ( Main/Exploration ); précédent : 002D80; suivant : 002D82Effects of R-(1-amino-2-phenylethyl)phosphonic acid on glyceollin accumulation and expression of resistance to Phytophthora megasperma f.sp. glycinea in soybean.
Auteurs : T. Waldmüller [Allemagne] ; H. GrisebachSource :
- Planta [ 0032-0935 ] ; 1987.
Abstract
(R)-(1-Amino-2-phenylethyl)phosphonic acid (R-APEP), an inhibitor of phenylalanine ammonia-lyase (PAL), was applied to the tap root of 42-h-old soybean (Glycine max. (L.) Merrill cv. Harosoy 63) seedlings during inoculation with zoospores of the incompatible race 1 of Phytophthora megasperma f.sp. glycinea (Pmg1) for 2 h and during a subsequent incubation period. In contrast to L-2-aminooxy-3-phenylpropionic acid, R-APEP was not toxic to the zoospores which remained virulent in presence of the inhibitor. A 50% inhibition of PAL activity in vitro was observed with 4.2 μM R-APEP and with 36 μM of the S-enantiomer. When R-APEP at 330 μM was applied for a total of 36 h to the seedlings, resistance against Pmg 1 was abolished. Such seedlings were indistinguishable in appearance from those seedlings which had been inoculated with the compatible race 3 of Pmg. Roots treated with R-APEP at 330 μM showed a reduction of about 47% in glyceollin content when measured 12 h after inoculation, and with 1 mM a 67% reduction. In contrast, treatment with S-APEP (1 mM) caused only a 20% reduction in glyceollin content. As determined by indirect immunofluorescence of fungal hyphae in cryotome cross-sections of roots, the growth pattern of the incompatible race 1 of Pmg changed to that of the compatible race 3 under conditions where R-APEP caused loss of resistance against Pmg 1. The results support the concept of an important role of glyceollin in resistance of soybean against incompatible races of the fungus.
DOI: 10.1007/BF00398673
PubMed: 24225928
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Waldmüller</name><affiliation wicri:level="3"><nlm:affiliation>Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II der Universität Freiburg, Schänzlestrasse 1, D-7800, Freiburg, Federal Republic of Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II der Universität Freiburg, Schänzlestrasse 1, D-7800, Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Grisebach, H" sort="Grisebach, H" uniqKey="Grisebach H" first="H" last="Grisebach">H. Grisebach</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1987">1987</date><idno type="RBID">pubmed:24225928</idno><idno type="pmid">24225928</idno><idno type="doi">10.1007/BF00398673</idno><idno type="wicri:Area/Main/Corpus">002D75</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002D75</idno><idno type="wicri:Area/Main/Curation">002D75</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002D75</idno><idno type="wicri:Area/Main/Exploration">002D75</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of R-(1-amino-2-phenylethyl)phosphonic acid on glyceollin accumulation and expression of resistance to Phytophthora megasperma f.sp. glycinea in soybean.</title><author><name sortKey="Waldmuller, T" sort="Waldmuller, T" uniqKey="Waldmuller T" first="T" last="Waldmüller">T. Waldmüller</name><affiliation wicri:level="3"><nlm:affiliation>Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II der Universität Freiburg, Schänzlestrasse 1, D-7800, Freiburg, Federal Republic of Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II der Universität Freiburg, Schänzlestrasse 1, D-7800, Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Grisebach, H" sort="Grisebach, H" uniqKey="Grisebach H" first="H" last="Grisebach">H. Grisebach</name></author></analytic><series><title level="j">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="1987" type="published">1987</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">(R)-(1-Amino-2-phenylethyl)phosphonic acid (R-APEP), an inhibitor of phenylalanine ammonia-lyase (PAL), was applied to the tap root of 42-h-old soybean (Glycine max. (L.) Merrill cv. Harosoy 63) seedlings during inoculation with zoospores of the incompatible race 1 of Phytophthora megasperma f.sp. glycinea (Pmg1) for 2 h and during a subsequent incubation period. In contrast to L-2-aminooxy-3-phenylpropionic acid, R-APEP was not toxic to the zoospores which remained virulent in presence of the inhibitor. A 50% inhibition of PAL activity in vitro was observed with 4.2 μM R-APEP and with 36 μM of the S-enantiomer. When R-APEP at 330 μM was applied for a total of 36 h to the seedlings, resistance against Pmg 1 was abolished. Such seedlings were indistinguishable in appearance from those seedlings which had been inoculated with the compatible race 3 of Pmg. Roots treated with R-APEP at 330 μM showed a reduction of about 47% in glyceollin content when measured 12 h after inoculation, and with 1 mM a 67% reduction. In contrast, treatment with S-APEP (1 mM) caused only a 20% reduction in glyceollin content. As determined by indirect immunofluorescence of fungal hyphae in cryotome cross-sections of roots, the growth pattern of the incompatible race 1 of Pmg changed to that of the compatible race 3 under conditions where R-APEP caused loss of resistance against Pmg 1. The results support the concept of an important role of glyceollin in resistance of soybean against incompatible races of the fungus. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24225928</PMID><DateCompleted><Year>2013</Year><Month>11</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0935</ISSN><JournalIssue CitedMedium="Print"><Volume>172</Volume><Issue>3</Issue><PubDate><Year>1987</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Effects of R-(1-amino-2-phenylethyl)phosphonic acid on glyceollin accumulation and expression of resistance to Phytophthora megasperma f.sp. glycinea in soybean.</ArticleTitle><Pagination><MedlinePgn>424-30</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/BF00398673</ELocationID><Abstract><AbstractText>(R)-(1-Amino-2-phenylethyl)phosphonic acid (R-APEP), an inhibitor of phenylalanine ammonia-lyase (PAL), was applied to the tap root of 42-h-old soybean (Glycine max. (L.) Merrill cv. Harosoy 63) seedlings during inoculation with zoospores of the incompatible race 1 of Phytophthora megasperma f.sp. glycinea (Pmg1) for 2 h and during a subsequent incubation period. In contrast to L-2-aminooxy-3-phenylpropionic acid, R-APEP was not toxic to the zoospores which remained virulent in presence of the inhibitor. A 50% inhibition of PAL activity in vitro was observed with 4.2 μM R-APEP and with 36 μM of the S-enantiomer. When R-APEP at 330 μM was applied for a total of 36 h to the seedlings, resistance against Pmg 1 was abolished. Such seedlings were indistinguishable in appearance from those seedlings which had been inoculated with the compatible race 3 of Pmg. Roots treated with R-APEP at 330 μM showed a reduction of about 47% in glyceollin content when measured 12 h after inoculation, and with 1 mM a 67% reduction. In contrast, treatment with S-APEP (1 mM) caused only a 20% reduction in glyceollin content. As determined by indirect immunofluorescence of fungal hyphae in cryotome cross-sections of roots, the growth pattern of the incompatible race 1 of Pmg changed to that of the compatible race 3 under conditions where R-APEP caused loss of resistance against Pmg 1. The results support the concept of an important role of glyceollin in resistance of soybean against incompatible races of the fungus. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Waldmüller</LastName><ForeName>T</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II der Universität Freiburg, Schänzlestrasse 1, D-7800, Freiburg, Federal Republic of Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grisebach</LastName><ForeName>H</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta</MedlineTA><NlmUniqueID>1250576</NlmUniqueID><ISSNLinking>0032-0935</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>1987</Year><Month>04</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>1987</Year><Month>06</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>11</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>1987</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1987</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24225928</ArticleId><ArticleId IdType="doi">10.1007/BF00398673</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Chem Biol Interact. 1970 Jun;2(1):53-5</Citation><ArticleIdList><ArticleId IdType="pubmed">5524009</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Cell Biol. 1983 Jul;31(1):167-9</Citation><ArticleIdList><ArticleId IdType="pubmed">6352265</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1976 May;57(5):751-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16659565</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1985 Mar;77(3):591-601</Citation><ArticleIdList><ArticleId IdType="pubmed">16664104</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1986 Jan;167(1):101-5</Citation><ArticleIdList><ArticleId IdType="pubmed">24241738</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1986 Apr;246(1):149-54</Citation><ArticleIdList><ArticleId IdType="pubmed">3963819</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Biochim Pol. 1985;32(2):131-43</Citation><ArticleIdList><ArticleId IdType="pubmed">4036447</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Fribourg-en-Brisgau</li></region><settlement><li>Fribourg-en-Brisgau</li></settlement></list><tree><noCountry><name sortKey="Grisebach, H" sort="Grisebach, H" uniqKey="Grisebach H" first="H" last="Grisebach">H. Grisebach</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Waldmuller, T" sort="Waldmuller, T" uniqKey="Waldmuller T" first="T" last="Waldmüller">T. Waldmüller</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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